Journal: Apmis

Article Title: Streptococcus mitis /human gingival fibroblasts co-culture: the best natural association in answer to the 2-hydroxyethyl methacrylate release

doi: 10.1111/j.1600-0463.2011.02828.x

Figure Lengend Snippet: Effect of 3 mM HEMA concentration on sessile (A) and planktonic (B) growth phases of Streptococcus mitis DS12 ( Sm DS12) and S. mitis ATCC 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts after 48 and 72 h of treatment.

Article Snippet: The treatment of S. mitis /HGFs with HEMA did not produce significant effects on the bacterial adhesion and induced an increase in planktonic S. mitis ATCC 6249 population after 48 and 72 h. HEMA increased significantly the planktonic S. mitis ATCC 6249 viability when co-cultured with HGFs, while a cytotoxic effect on HGFs, without bacteria, was recorded.

Techniques: Concentration Assay, Cell Culture

Journal: Apmis

Article Title: Streptococcus mitis /human gingival fibroblasts co-culture: the best natural association in answer to the 2-hydroxyethyl methacrylate release

doi: 10.1111/j.1600-0463.2011.02828.x

Figure Lengend Snippet: Effect of 3 mM 2-hydroxyethyl methacrylate (HEMA) concentration on the viability of Streptococcus mitis DS12 ( Sm DS12) and S. mitis ATCC 6249 ( Sm ATCC) alone and co-cultured on human gingival fibroblasts (HGFs) after 48 and 72 h of treatment. (A) Representative images of sessile streptococcal strains alone (rows 1 and 3) and co-cultured with HGFs (rows 2 and 4) untreated and treated with HEMA. Arrows indicate the bacterial aggregation on HGFs. Images showed in inserts represent the corresponding planktonic phases in which the HEMA treatment produces a significant increase of bacterial viability with respect to the control (see histograms; B). Live/dead staining, scale bar = 10 μm; (B) percentage of streptococcal viability on sessile and planktonic growth phases, untreated and treated with HEMA.

Article Snippet: The treatment of S. mitis /HGFs with HEMA did not produce significant effects on the bacterial adhesion and induced an increase in planktonic S. mitis ATCC 6249 population after 48 and 72 h. HEMA increased significantly the planktonic S. mitis ATCC 6249 viability when co-cultured with HGFs, while a cytotoxic effect on HGFs, without bacteria, was recorded.

Techniques: Concentration Assay, Cell Culture, Control, Staining

Journal:

Article Title: Photoactive Porphyrin Derivative with Broad-Spectrum Activity against Oral Pathogens In Vitro

doi:

Figure Lengend Snippet: Oral bacteria tested

Article Snippet: All the streptococcal strains and Actinomyces viscosus were grown at 35°C in a Brewer jar with a GasPak carbon dioxide generator (Becton Dickinson, Cockeysville, Md.), which created an aerobic atmosphere with approximately 10% carbon dioxide. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Organism Strain Source Medium a Porphyromonas gingivalis ATCC 33277 ATCC b HK Porphyromonas gingivalis 7-1-4 (patient isolate) A. Tanner HK Bacteroides forsythus ATCC 43037 ATCC NAM Fusobacterium nucleatum subsp. polymorphum ATCC 10953 ATCC TSBY Campylobacter rectus ATCC 33238 ATCC FF Eikenella corrodens ATCC 23834 ATCC TSBY Actinobacillus actinomycetemcomitans ATCC 29523 ATCC Chocolate Actinomyces viscosus ATCC 15987 ATCC BHI Streptococcus sobrinus ATCC 6715 ATCC TSBY Streptococcus mitis ATCC 15914 ATCC TSBY Streptococcus oralis ATCC 35037 ATCC TSBY Streptococcus mutans ATCC 25175 ATCC TSBY Open in a separate window a HK, hemin-menadione; NAM, N -acetylmuramic acid; TSBY, tryptic soy brain heart infusion yeast extract; FF, Fumarate and formate medium (brain heart infusion agar, 52 g/liter; yeast extract, 10 g/liter; sodium formate, 2 g/liter; disodium fumarate, 4.1 g/liter; hemin, 5 g/liter; 50 ml of sheep red blood cells [pH 7.5]); Chocolate, chocolate agar; BHI, brain heart infusion agar. b ATCC, American Type Culture Collection.

Techniques: Bacteria, Chocolate

Journal:

Article Title: Photoactive Porphyrin Derivative with Broad-Spectrum Activity against Oral Pathogens In Vitro

doi:

Figure Lengend Snippet: Susceptibilities of oral bacteria to photosensitizers ce6 and ce6-5K a

Article Snippet: All the streptococcal strains and Actinomyces viscosus were grown at 35°C in a Brewer jar with a GasPak carbon dioxide generator (Becton Dickinson, Cockeysville, Md.), which created an aerobic atmosphere with approximately 10% carbon dioxide. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Organism Strain Source Medium a Porphyromonas gingivalis ATCC 33277 ATCC b HK Porphyromonas gingivalis 7-1-4 (patient isolate) A. Tanner HK Bacteroides forsythus ATCC 43037 ATCC NAM Fusobacterium nucleatum subsp. polymorphum ATCC 10953 ATCC TSBY Campylobacter rectus ATCC 33238 ATCC FF Eikenella corrodens ATCC 23834 ATCC TSBY Actinobacillus actinomycetemcomitans ATCC 29523 ATCC Chocolate Actinomyces viscosus ATCC 15987 ATCC BHI Streptococcus sobrinus ATCC 6715 ATCC TSBY Streptococcus mitis ATCC 15914 ATCC TSBY Streptococcus oralis ATCC 35037 ATCC TSBY Streptococcus mutans ATCC 25175 ATCC TSBY Open in a separate window a HK, hemin-menadione; NAM, N -acetylmuramic acid; TSBY, tryptic soy brain heart infusion yeast extract; FF, Fumarate and formate medium (brain heart infusion agar, 52 g/liter; yeast extract, 10 g/liter; sodium formate, 2 g/liter; disodium fumarate, 4.1 g/liter; hemin, 5 g/liter; 50 ml of sheep red blood cells [pH 7.5]); Chocolate, chocolate agar; BHI, brain heart infusion agar. b ATCC, American Type Culture Collection.

Techniques: Bacteria, Control